...sformantswereobtained2weeksafterselectionwithG418orhygromycinandscreenedin[3H]DAMGOor[3H]diprenorphine-bindingassays(PerkinElmerLifeandAnalyticalSciences).RelativeSemiquantitativeRT-PCR.TotalRNAobtainedfrommousebrainasdescribedabovewastreatedwithTURBODNA-freereagents(Ambion,Austin,TX)andreverse-tran...
参考资料医源资料库；在线期刊；分子药理学杂志；2006年第68卷第3期...).Likewise,RTVhamperedtheplasmaclearanceofintravenouslyinjectedglyceroltri[3H]oleate-labeledVLDL-likeemulsionparticles(halftime,19.3±10.5versus5.0±1.3minutesincontrols;P0.05)associatedwithadecreaseof44%inplasmalipoproteinlipaseactivity.Accordingly,RTVdecreasedtheuptakeofTG-derivedfatty...
参考资料医源资料库；在线期刊；动脉硬化血栓血管生物学杂志；2006年第26卷第1期...ys,suggestingthatBSDLisinvolvedinangiogenesis.MaterialsandMethodsMaterials[3H]-thymidine(5Ci/mmol),[125I]-bFGF(2958Ci/mmol),and[125I]-VEGF(4484Ci/mmol)camefromPerkinElmerLifeSciences(Courtaboeuf,France).Fluoresceinisothiocyanate-BSDLwaspreparedasdescribed.3Polyclonalantibodies(pAbL64)againsthumanpan...
参考资料医源资料库；在线期刊；动脉硬化血栓血管生物学杂志；2005年第25卷第2期...nenucleosidesandincreasedVmaxvaluesforpurinenucleosides.CysorSeratposition33hadsimilareffectsonthekineticparametersofhENT2asMet,indicatingthathydrophobic(MetandCys)orhydrogen-bondingenergy(Ser)contributedtopermeantbindingbytheseresidues.hENT2-I33MandI33Cdisplayedincreasedsensitivitiestodipyridamolec...
参考资料医源资料库；在线期刊；分子药理学杂志；2005年第67卷第4期...放开，恢复血流灌注1h，7只;（5）亚硒酸钠+脑缺血再灌注3h组（Se+IR3h）动脉夹夹住两侧颈总动脉30min后放开，恢复血流灌注3h，7只。Se+IR1h、Se+IR3h组均于实验前4天开始腹腔注射亚硒酸钠，2mg/（kg·d）。SAM、IS、IR组分别注射等体积...
参考资料合作平台；在线期刊；中华现代中西医杂志；2005年第3卷第6期；论著...ureonthebasisofthetransporter‘sinteractionswithitssubstrate.Materials.[2-3H]Adenosine(0.92TBq/mmol),[5,6-3H]uracil(1.78TBq/mmol),and[8-3H]-hypoxanthine(1.18TBq/mmol)werepurchasedfromAmershamPharmaciaBiotech(LittleChalfont,Buckinghamshire,UK).[2,8-3H]-Adenine(1.19TBq/mmol)wasboughtfromPerkinElmerLi...
参考资料医源资料库；在线期刊；分子药理学杂志；2007年第69卷第3期...射5min组、注射20min组。利用活体肾小管显微穿刺技术，将3H-庆大霉素或庆大霉素分别注入实验组和对照组近端小管腔内，常规制作光镜、电镜放射自显影切片，显微镜观察、摄影并进行图像分析。结果光镜放射自显影显示:3H-庆...
参考资料合作平台；在线期刊；中华中西医杂志；2005年第6卷第6期；论著...GBq/mmol),[14C]creatinine(2.035GBq/mmol),[14C]procainamide(2.035GBq/mmol),[3H]quinidine(740GBq/mmol),[3H]quinine(740GBq/mmol),L-[N-methyl-3H]carnitine(3.145TBq/mmol),and[N-methyl-14C]nicotine(2.035GBq/mmol)wereobtainedfromAmericanRadiolabeledChemicals(St.Louis,MO).[14C]metformin(962MBq/mmol),[14C]gu...
参考资料医源资料库；在线期刊；美国生理学杂志；2007年第290卷第2期【摘要】Objective—C3H/HeJ(C3H)miceareextremelyresistanttoatherosclerosis.Toidentifythegeneticfactorsinvolvedinlesioninitiation,westudiedacrossbetweenC3HandthesusceptiblestrainC57BL/6J(B6)onahyperlipidemic(apolipoproteinE–null)background.MethodsandResults—Whereasapreviouscrossin...
参考资料医源资料库；在线期刊；动脉硬化血栓血管生物学杂志；2007年第27卷第12期...gy(LakePlacid,NY)andCHEMICONInternationalInc.(Temecula,CA),respectively.L-[3H]Carnitine(3.1TBq/mmol)waspurchasedfromAmershamBiosciences(LittleChalfont,Buckinghamshire,UK).[14C]Inulin(89MBq/g)wasfromPerkinElmerLifeSciences(Boston,MA).Hyaluronidase(bovinetestisType1-S)waspurchasedfromSigmaChemicalCo.(...
参考资料医源资料库；在线期刊；分子药理学杂志；2006年第68卷第9期