...冰上放置30min。42℃加热90s后,冰上1min。再加入890μlSOC培养基,37℃振荡培养60min,8000r/min离心5min,弃上清900μl,将菌液混匀,取100μl涂板在含有XGal、IPTG、Amp的L琼脂平板培养基上培养,形成单菌落,挑选白色菌落,使...