...ibodywaspurchasedfromCALTAG.ThesemAbsweredirectlycoupledtoFITC,PE,Cy-crome,APC,orbiotin.SurfacemarkerexpressiononthymocytesandperipheralTcellswasvisualizedusingaFACScalibur(BectonDickinson)andanalyzedwithFlowJo(TreeStar)andCellQuestsoftware(BecktonDickinson).CellsortingwasperformedusingMo-Flo(DakoCy...
参考资料医源资料库;在线期刊;实验医学杂志;2006年第203卷第6期...and-c)andgroupII(CD1d;reference12).TheCD1proteinsareexpressedonprofessionalAPCs(13),andcanpresentbacterialorsyntheticlipidsandglycolipidstovariousTcellsubpopulations(14,15),whichinturnmediateeffectorfunctionsincludingcytolysisandIFN-secretionthatareknowntobeprotectiveforthehost(16).Currently,itisnot...
参考资料医源资料库;在线期刊;实验医学杂志;2005年第202卷第2期...bodieswereused:anti-CD19-FITCorPE-Cy7;anti-CD8-PE;anti-CD4-PE-Cy5;anti-CD3-APC;anti-CD5-PE;anti-CD45R(B220)-PE-Cy5orPerCPCy5.5;anti-CD11b-APC;anti-I-Ad-FITCorPE;anti-CD11c-PEorAPC;anti-CD86-biotin;andanti-CD40-biotin(allfromBDBiosciences,SanJose,CA),anti-CD-11b-PacificBlue(CaltagLaboratories,Burling...
参考资料医源资料库;在线期刊;美国病理学杂志;2006年第168卷第4期...anJournalofPodiatricMedicine》创刊于1997年,为澳洲足病委员会(APC)的官方杂志,由《AustralianPodiatrist》和《NewZealandJournalofPodiatricMedicine》合并而成,每年三期。《BritishJournalofPodiatry》由欧洲手足医师协会主办。《JournalofFootandAnkleResearch...
参考资料医源资料库;在线期刊;中国矫形外科杂志;2009年第17卷第22期...的小鼠,在雷帕霉素诱导的自噬作用下,抗原呈递细胞(APCs)表达Ag85B增强。此外,雷帕霉素使结核分枝杆菌局限于自噬和溶酶体。3–甲基腺嘌呤或beclin-1小分子干扰RNA可抑制自噬,减弱雷帕霉素增强抗原递呈。因此可见,卡介苗...
参考资料行业资讯;临床快报;流行病与传染病...sus-sidescatter(FSC-versus-SSC)profilesofPBMCs(leftpanel).ContaminatingCD3-APC+Tlymphocytesweregatedoutofthemonocytepopulation(middlepanel).CD14highCD16lowandCD14lowCD16highmonocyteacquisitiongateswereset(rightpanel),and5x104to3x105cellspermonocytepopulationweresorted.Figure6Expansionofmonocytepopul...
参考资料医源资料库;在线期刊;临床研究杂志;2005年第115卷第9期...mposedofV9-C1andV2chainsisrequiredforrecognitionofDaudicellsorforZOL-pulsedAPCs.TCellStimulationAssays.CellsusedasstimulatoryAPCswereincubatedwithdifferentdosesofbisphosphonatesfor3hat37°Corat4°Cfollowedbythreewashesbeforeplating(5x104/well)andsubsequentadditionofTcells(5x104/well).Insomeexperimen...
参考资料医源资料库;在线期刊;美国病理学杂志;2003年第162卷第2期...mposedofV9-C1andV2chainsisrequiredforrecognitionofDaudicellsorforZOL-pulsedAPCs.TCellStimulationAssays.CellsusedasstimulatoryAPCswereincubatedwithdifferentdosesofbisphosphonatesfor3hat37°Corat4°Cfollowedbythreewashesbeforeplating(5x104/well)andsubsequentadditionofTcells(5x104/well).Insomeexperimen...
参考资料医源资料库;在线期刊;实验医学杂志;2003年第197卷第2期...ipidbutratherbyglycosaminoglycanspresentonthecellsurface.ActivatedproteinC(APC)activityisacutelysensitivetothemembraneenvironment,butithasbeenshownthatlipidalterationsimpactAPCactivityandprothrombinaseactivityindifferentways.Smirnovetal,34forexample,showedthatlupusanticoagulantimmunoglobulininhibite...
参考资料医源资料库;在线期刊;动脉硬化血栓血管生物学杂志;2007年第27卷第3期...用树突状细胞(DC)是免疫系统最有效的抗原递呈细胞(APC)。DC表面的MHC分子能与肿瘤抗原相结合,从而使T细胞识别抗原。DC表面B7分子能给T细胞提供协同刺激信号。这两种功能共同作用激活T细胞,产生抗肿瘤免疫。而肿瘤浸...
参考资料合作平台;在线期刊;中华实用医药杂志;2004年第4卷第24期;综述