...Statisticswasperformedonsetsofthreeexperimentsandrevealedsignificancewithap0.05.D,DMSOtreatment;A,androstenedione;Preg,pregnenolone;17OHPreg,17-hydroxypregnenolone.ProteinAnalyses.CellproteinextractionsandWesternblotanalysisofERK1/2andMEKphosphorylationandPPARexpressionwereperformedaccordingtothepro...
参考资料医源资料库；在线期刊；分子药理学杂志；2007年第69卷第3期...0mMNH4ClinPBSfor30minfollowedby3x10minwithPBSblockingbuffercontaining1%BSA,0.05%saponin,and0.2%gelatin.Thesectionswereincubatedwithprimaryantibody(dilutedinPBSwith0.1%BSAand0.3%TritonX-100)overnightat4°C.Thesectionswerewashed3x10minwithPBSwash-buffercontaining0.1%BSA,0.05%saponin,and0.2%gelatina...
参考资料医源资料库；在线期刊；美国生理学杂志；2004年第287卷第5期...mSP.DensitometricanalysiswasperformedusingAlphaInnotechAlphaEaseFCversion4.0.0software.TheratioofphosphoproteintototalproteininthepresenceofWIN55,212-2alonewassetat1,andallvalueswereexpressedrelativetothatvalue.ImmunofluorescenceandConfocalMicroscopyCHO-K1cellstransfectedwithaconstructcontainingtheC...
参考资料医源资料库；在线期刊；分子药理学杂志；2006年第68卷第7期...erbyStudent‘sttestorbyone-wayANOVA,followedbyaNewman-Keulstest.AvalueofP<0.05wasconsideredstatisticallysignificant.ConfirmationofSAGEResults.Gene-expressionprofilingwasusedtoidentifythespectrumofgenesregulatedbyaconstitutivelyactiveHIF-1hybrid,HIF-1/VP16,andtocomparethesewiththegenesregulatedbyhyp...
参考资料医源资料库；在线期刊；分子药理学杂志；2006年第68卷第6期...C.T.compound(Sakura,Tokyo,Japan)werefixedinacetoneandblockedwith0.5%casein/0.05%thimerosalinPBS.Forimmunostainingß6onhumantissue,sectionswereincubatedwithmurine2A123andananti-mouseAlexaFluor594secondaryantibody(A-11032;MolecularProbes,Eugene,OR).Forimmunostainingß6onmousetissue,sectionsw...
参考资料医源资料库；在线期刊；美国病理学杂志；2007年第169卷第1期...swereconsideredstatisticallysignificantwhentheprobabilityvalueswerelessthan0.05.ResultsTransientIschemiaInducesNeuronalCellApoptosisintheRetinaToexaminetheneuroprotectiveeffectsofVEGF-A,weusedtheretinalI/Rmodel.Thetimecourseofapoptosisintheretinawasfirstcharacterizedafter60minutesoftransientischemia...
参考资料医源资料库；在线期刊；美国病理学杂志；2007年第169卷第7期...Methods.DifferencesbetweenmeanswerecomparedbyANOVAandbyStudent‘sttests.P<0.05wasconsideredstatisticallysignificant.Resultsarepresentedasmeans±S.D.Effectsof5-AEDonHumanCD34+CellSurvival,Differentiation,andClonogenicityafterIR.Inpilotstudies,5-AEDupto10µg/mlhadnotoxicity,andtheoptimaldo...
参考资料医源资料库；在线期刊；分子药理学杂志；2007年第69卷第8期...肪12.7g21%碳水化合物70.6g24%钠204mg10%维生素A72mgRE9%维生素B10.09mg6%（十八）“营养成分表与包装的基线垂直应如何理解？包装的基线是指包装的直线边缘或轴线，或者是产品的底面形成的基线。在保证营养成分表为方框表的前提...
健康行业资讯；食品安全；政策调整...oformandoncewithchloroform,andprecipitatedinthepresenceof0.3Msodiumacetate/0.01MMgCl2in5Volofethanolat-80°Cfor30min.TheDNApelletsweredissolvedin50µlofelutionbuffer(10mMTris·HCl,pH8.5).PCRamplificationofa186-bpfragmentoftheNHE3promotercontaininganSpconsensuselementwascarriedoutusingp...
参考资料医源资料库；在线期刊；美国生理学杂志；2006年第289卷第5期...ysisAlldatawereanalyzedusingone-wayanalysisofvarianceorunpairedt-test,andP<0.05orlowerwasconsideredtobesignificant.ResultsIntroductionofPS1M146VAlleleDoesNotAlterTransgeneExpressionandSteady-StateLevelsTotestthephenotypicconsequencesofaugmentingAß42levelsinskeletalmuscle,weusedageneticapproach...
参考资料医源资料库；在线期刊；美国病理学杂志；2006年第168卷第6期