...rdandreversegene-specificprimer(Table1),1.5xRT-PCRbuffer,1xadditivereagent(0.004mgofbovineserumalbumin,150mMtrehalose,and0.2%Tween20;Cepheid,Sunnyvale,CA),250µMdNTPs,3mMMg2+,0.25xSYBRgreendye(CambrexBioScienceRockland,Inc.,RocklandME),and2.5UofExTaqHSpolymerasewerecombinedandbroughtto25µ...
参考资料医源资料库；在线期刊；分子药理学杂志；2007年第69卷第6期...olvingfireflylanternextractinareactionbuffer,pH7.4,containing0.33MK3AsO4and0.02MMgSO4.Cultureswereharvestedinlysisbuffer,pH7.4,containing25mMTris,1mMEGTA,1mMEDTA,1%TritonX-100,and15%glycerol.Sampleswerecentrifugedat14,000gfor10min,andATPwasmeasuredinthesupernatantunderlinearrateconditions.Samples(10...
参考资料医源资料库；在线期刊；分子药理学杂志；2006年第68卷第5期...erewashedonetimewithHanks‘balancedsaltsolution,1.0g/lD-glucose,0.4g/lKCl,0.06g/lKH2PO4,8.0g/lNaCl,0.05g/lNa2HPO4,0.01g/lphenolred,and0.35g/lNaHCO3.Cellswerethenincubatedwith0.05mg/mlneutralredsolutionfor1hat37°C/5%CO2.Theneutralredsolutionwasaspirated,and1ml1%fomaldehyde/1%CaCl2solution(wash-f...
参考资料医源资料库；在线期刊；美国生理学杂志；2004年第287卷第12期...umdeprivation.Inthesecells,24hofserumstarvationincreasedpHifrom7.08±0.008(n=34)to7.18±0.018(n=33)aswellasthepHrecoveryratefromintracellularacidificationwithNH4Clfrom0.29±0.022pHU/min(n=14)to0.50±0.024pHU/min(n=14),withoutmodifyingtheirbufferingcapacity.Theseeffectswerefol...
参考资料医源资料库；在线期刊；美国生理学杂志；2006年第289卷第7期...cellularANGIIwasinhibitedbytheAT1receptorblockerlosartan(16.5±4.6%,P0.01vs.ANGII,78.3±6.2%)andbythetyrosinephosphataseinhibitorphenylarsineoxide(PAO;30.0±3.5%,P0.05vs.ANGII).IntracellularANGIIlevelswereincreasedby58%(basal,229.8±11.4vs.ANGII,361.3±11.8pgANGII/mgpro...
参考资料医源资料库；在线期刊；美国生理学杂志；2006年第289卷第8期...n;S.E.M.unlessotherwisespecified.Forsignificanceofdifferences,*representsp<0.05and**representsp<0.01;statisticalanalysiswasconductedusingStudent‘sttest(unpaired).Clampfitsoftware(MolecularDevices)wasusedtoanalyzecurrenttraces.Datafromeachtestpulse(currentsinthepresenceofdrug)werenormalizedtocontro...
参考资料医源资料库；在线期刊；分子药理学杂志；2007年第69卷第12期...inglecultureofhumanhepatocytes(C)expressedrelativetocontrolhepatocytes.*,p<0.05,significantlyfromcontrols.,p<0.05significantlyfromcellstreatedwith100nMDEX.ChromatinImmunoprecipitationAssay.Humanhepatocyteswereculturedintheabsenceofglucocorticoidsfor16handthentreatedwith100nMDEXfor6h.Forexperimentswi...
参考资料医源资料库；在线期刊；分子药理学杂志；2008年第70卷第2期...果后者明显促进肠癌发生率，与前者相比显著差异（P＜0.0005）。但如在给致癌物这前或同时给高脂饲料，肿瘤发生数则无明显差别。 关于胆固醇，Reddy和Watanabe实验了石胆酸钠、胆固醇、胆固醇5α，6α-环氧化物、胆石烷-3β...
参考资料医源资料库；医源图书馆；教材类；临床营养学...with50µlofelutionbuffercontaining80mMDTT,0.24mMTris·HCl,pH8.9,0.008%bromophenolblue,5.6%SDS,and16%glycerol.TheproteinsweresubjectedtoSDS-PAGE,transferredontoanitrocellulosemembrane,andblottedwithanti-NBC1oranti-V5antibody.Basolateralmembranelabeling.OKcellsweregrownon0.45-µmpolyca...
参考资料医源资料库；在线期刊；美国生理学杂志；2006年第289卷第9期...realistic.Thishasnowbeendone:thecellmembraneCO2permeabilityhasbeenreducedto0.012cm/s(8%ofthevalueusedinRef.23),andwiththisreduction,thetubuleCO2permeabilityisnow0.014cm/s.ThismaybecomparedwiththeproximaltubuleCO2permeabilitymeasuredbySchwartzetal.(36)of10-4cm2/s,orfora25-µm-diametertubule0.013...
参考资料医源资料库；在线期刊；美国生理学杂志；2007年第290卷第4期